TABLE 3.
A260/A280 Ratios of Four Kits (by gender)
Kit/gender | Mean ratio A260/A280 (±SD) | Possible confounding factors |
---|---|---|
Qv Woman | 3.33 ± 0.12 | carrier RNA, sodium azide |
Qv Man | 3.39 ± 0.06 | |
Qm Woman | 2.53 ± 0.34 | carrier RNA, sodium azide |
Qm Man | 2.48 ± 0.64 | |
iG Woman | 1.19 ± 0.60 | residual chemicals (phenol, guanidine) or other reagent used in the extraction protocol |
iG Man | 1.64 ± 0.19 | |
Ab Woman | 0.93 ± 0.39 | residual chemicals (phenol, guanidine) or other reagent used in the extraction protocol and/or a very low DNA concentration |
Ab Man | 0.96 ± 0.16 |
Pure DNA has a A260/A280 ratio of 1.8.