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. 2014 Dec;25(4):96–110. doi: 10.7171/jbt.14-2504-002

TABLE 3.

A260/A280 Ratios of Four Kits (by gender)

Kit/gender Mean ratio A260/A280 (±SD) Possible confounding factors
Qv Woman 3.33 ± 0.12 carrier RNA, sodium azide
Qv Man 3.39 ± 0.06
Qm Woman 2.53 ± 0.34 carrier RNA, sodium azide
Qm Man 2.48 ± 0.64
iG Woman 1.19 ± 0.60 residual chemicals (phenol, guanidine) or other reagent used in the extraction protocol
iG Man 1.64 ± 0.19
Ab Woman 0.93 ± 0.39 residual chemicals (phenol, guanidine) or other reagent used in the extraction protocol and/or a very low DNA concentration
Ab Man 0.96 ± 0.16

Pure DNA has a A260/A280 ratio of 1.8.