Fig. 2.

Quercetin-generated ROS strongly induced apoptosis and expressed Sestrin 2, p53 as well as activated AMPKα 1. (A) Cells were treated with indicated concentration of H₂O₂ and quercetin for 24 h. Cells were stained with Annexin V/PI and fluorescence intensity was measured by flow cytometer. (B) Cells were treated with indicate concentration of H₂O₂ and quercetin for 6 h. Next, expression of Sestein 2, p53 and activate of AMPKα1, mTOR were analyzed by Western blotting.