Fig. 4.

Kaempferol suppresses the activity of Cdc2 in HT-29 cells. Cells were treated with 0 or 60 μmol/L kaempferol for the indicated periods. (A) Cell lysates were analyzed via Western blotting with the indicated antibodies. (B) Cell lysates were incubated with anti-Cdc2 antibody, and the immunoprecipitated proteins were prepared as described in Fig. 3B. The precipitated proteins were analyzed by an in vitro kinase assay using Histone H1 (HH1) as a substrate or by Western blotting. The photographs of results, which are representative of two or three independent experiments, are shown.