Fig. 2.

Effects of linoleic acid on levels of COXs expression in AGS cells. (A) After treatment with linoleic acid for 96 h, total RNA was isolated, and RT-PCR was performed using the indicated primers. The amplified PCR products were run in a 1% agarose gel and visualized by EtBr staining. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a housekeeping control gene. (B) Cells grown under the same conditions as (A) were collected, lysed and cellular proteins were separated on SDS-poly-acrylamide gels and transferred onto nitrocellulose membranes. The membranes were probed with the indicated antibodies. Proteins were visualized using the ECL detection system. Actin was used as a loading control.