Figure 7.

Large airway epithelial AREG protein is overexpressed in DA-induced bronchiolitis obliterans (BO). Rodent tissue was assessed for collagen using Masson trichrome staining (A and B), AREG (green) (C–F), smooth muscle actin (SMA; red) (C and D), and β-catenin (red) (E and F), and nuclear counterstained with 4′,6-diamidino-2-phenylindole (blue) 7 days after DA exposure. Control airways (A, C, and E) illustrate minimal collagen, AREG, and SMA expression and junctional β-catenin within the airway regions. Fibrotic airways from a DA-treated rodent (B, D, and F) express increased collagen, AREG, and SMA expression within the airway epithelium. Magnification of all images is 200×.