Figure 3.

Mucus viscosity affects CBF. HBE monolayers were treated with four different viscosity standards at 6 centipoises (cPs), 55 cPs, 150 cPs, and 400 cPs, 10 μl on the apical surface. Cells were imaged by micro-optical coherence tomography (μOCT) at baseline and 15 minutes after addition of the standard. CBF measured in untreated cells (open circles), and cells pretreated with 1,2-Bis(2-aminophe1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)noxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM) (100 μM, filled circles). *P < 0.05 compared with baseline within condition. Data presented are means (± SEM).