Table 4.
mRNA Gene Expression by Real-Time PCR in Murine Lung Epithelial 12 Cells at 12 and 24 Hours of Room Air versus Hyperoxia Exposure
| Gene | 12 Hours |
24 Hours |
||||
|---|---|---|---|---|---|---|
| RA (n = 3) | O2 (n = 3) | P Value | RA (n = 3) | O2 (n = 3) | P Value | |
| Nrf2-mediated antioxidant genes (FC) | ||||||
| Ho-1 | 1.39 ± 0.09 | 1.61 ± 0.01 | NS | 1.00 ± 0.02 | 2.15 ± 0.12 | <0.001 |
| Gclc | 1.53 ± 0.10 | 1.41 ± 0.05 | NS | 1.02 ± 0.02 | 1.27 ± 0.10 | NS |
| Nqo-1 | 1.48 ± 0.05 | 1.71 ± 0.08 | NS | 0.77 ± 0.04 | 1.69 ± 0.08 | <0.001 |
| SOD antioxidant genes (FC) | ||||||
| SOD1 | 1.63 ± 0.02 | 2.36 ± 0.11 | 0.002 | 1.43 ± 0.02 | 2.06 ± 0.13 | 0.009 |
| SOD2 | 1.76 ± 0.03 | 1.54 ± 0.20 | NS | 1.24 ± 0.03 | 1.83 ± 0.21 | 0.009 |
| SOD3 | 1.00 ± 0.04 | 0.57 ± 0.07 | 0.006 | 1.00 ± 0.09 | 0.71 ± 0.08 | 0.01 |
Definition of abbreviations: FC, fold change; Gclc, glutamate-cysteine ligase catalytic subunit; Ho-1, heme oxygenase-1; Nrf2, nuclear factor erythroid 2; NS, not significant; Nqo-1, NAD(P)H dehydrogenase [quinine]-1; O2, hyperoxia; RA, room air; SOD, superoxide dismutase.