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. 2014 Oct 1;7:71. doi: 10.1186/s13041-014-0071-0

Figure 3.

Figure 3

The downregulatory effects of let-7a, miR-9, and miR-129-5p are mediated via specific sequences in the human FOXP2 3′ UTR. (A) miR-9, miR-19b, miR-140-5p, miR-200a, let-7a, miR-129-5p, miR-582-5p, and miR-892a significantly repressed luciferase activity when co-transfected with a reporter construct containing a wild-type FOXP2 3′ UTR, but had no effects when co-transfected with a reporter construct containing no 3′ UTR. (B) Mutations in the binding sites for let-7a, miR-9, and miR-129-5p in the human FOXP2 3′ UTR. Mutated nucleotides are in lower case letters. (C) Mutations in both the two binding sites for let-7a, miR-9, and miR-129-5p in the FOXP2 3′ UTR abolished the repressive effects of these miRNAs in luciferase reporter assays. Transfection was performed three times (n = 3) for each miRNA, and luciferase assays were performed in duplicate. To control for transfection efficiency, Renilla luciferase activity was normalized to the internal control firefly luciferase activity. Error bars = SEM; *p < 0.05, **p < 0.01, and ***p < 0.001, Tukey’s “Honest Significant Difference” test.