Table 2.
Description of the 4 control and 60 M.tb recombinant antigens tested, included DosR regulon-encoded (n = 6), TB reactivation-associated (n = 12), Rpf (n = 1), starvation (n = 1), other stress response-associated (n = 6), and IVE-TB antigens (n = 34) (Function information source: http://www.ncbi.nlm.nih.gov/nuccore).
| Antigen name | Function |
|---|---|
| CONTROL ANTIGENS (n = 4) | |
| PPD | Purified protein derivative |
| Rv0288 (TB10.4) | Low molecular weight protein antigen belongs to the ESAT-6 (esx) family |
| Rv3875/3874 (ESAT-6/CFP-10) | 6-kDa early secretory antigenic target/10 kDa culture filtrate (fusion protein) |
| Rv3804c (Ag85A) | Secreted antigen. Fibronectin binding protein acyltransferase activity |
| M.tb RECOMBINANT ANTIGENS (n = 60) | |
| DosR | |
| Rv0570c | Probable ribonucleoside-diphosphate reductase C-ter (aa 333-692) |
| Rv0570n | Probable ribonucleoside-diphosphate reductase N-ter (aa 1-354) |
| Rv1733 | Probable conserved transmembrane protein |
| Rv2626 | Conserved hypothetical protein |
| Rv2627 | Conserved hypothetical protein |
| Rv2628 | Hypothetical protein |
| Reactivation | |
| Rv0140 | Conserved hypothetical protein |
| Rv0251 | Possible heat shock protein |
| Rv0384 | Heat shock protein F84.1 |
| Rv0753 | Methylmalmonate semialdehyde dehydrogenase |
| Rv1471 | Thioredoxin reductase |
| Rv1874 | Hypothetical protein |
| Rv1875 | Conserved hypothetical protein |
| Rv2465* | Phosphopentose isomerase |
| Rv2466 | Conserved hypothetical protein |
| Rv2662 | Hypothetical protein |
| Rv3223 | ECF subfamily sigma subuint |
| Rv3862 | Possible transcriptional regulatory protein WHIB6 |
| Rpf | |
| Rv2389 | Possible resuscitation promoting factor D |
| Starvation | |
| Rv2660 | Hypothetical protein |
| Other M.tb stress induced | |
| Rv0244 | Probable Acyl-coA dehydrogenase |
| Rv0767 | Conserved hypothetical protein |
| Rv1909 | Ferric uptake regulation protein |
| Rv2745 | Possible transcriptional regulatory protein |
| Rv2913 | Possible D-amino acid amonohydrolase |
| Rv3406 | Probable dioxygenase |
| IVE-TB | |
| Rv0847 | Probable LpqS, lipoprotein |
| Rv0967 | Copper-sensitive operon repressor |
| Rv0990 | Hypothetical protein |
| Rv0991 | Conserved serine rich protein |
| Rv1170 | N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase MshB |
| Rv1284*≠ | Conserved hypothetical protein |
| Rv1363 | Possible membrane protein |
| Rv1403 | Putative methyltransferase |
| Rv1806 | PE family protein PE20 |
| Rv1955 | Possible toxine HigB |
| Rv1956*≠ | Possible antitoxin HigA |
| Rv1957 | Hypothetical protein |
| Rv2034*≠ | ArsR repressor protein |
| Rv2035 | Conserved hypothetical protein |
| Rv2225 | 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) |
| Rv2324* | Probable transcriptional regulatory protein (probably AsnC-family) |
| Rv2380c | Peptide synthetase mbtE C-ter (aa 1120-1682) |
| Rv2380M | Peptide synthetase mbtE middle part (aa 560-1140) |
| Rv2380N | Peptide synthetase mbtE N-ter (aa 1-580) |
| Rv2435c | Probable cyclase (adenylate or guanylate cyclase) C-ter (aa 340-730) |
| Rv2435n | Probable cyclase (adenylate or guanylate cyclase) N-ter (aa 1-360) |
| Rv2558 | Conserved protein |
| Rv2642 | Possible transcriptional regulatory protein |
| Rv2643 | Probable arsenic-transport integral membrane protein ArsC |
| Rv2658 | Possible prophage protein |
| Rv2737c | Recombination protein recombinase A (recA) C-ter (aa 400-790) |
| Rv2737n | Recombination protein recombinase A (recA) N-ter (aa 1-420) |
| Rv2838 | Probable ribosome-binding factor A (P15B protein) |
| Rv2982 | Probable glycerol-3-phosphate dehydrogenase (gpdA2) |
| Rv3353 | Conserved hypothetical protein |
| Rv3420 | Ribosomal-protein-alanine acetyltransferase rimI |
| Rv3515* | Fatty-acid-CoA synthase |
| Rv3536 | Probable hidratase |
| Rv717 | 30S ribosomal protein S14 RpsN1 |
M.tb recombinant antigens with Rv designation in bold induced a relevant IFN-γ response in this study.
*
also EHR.
≠
also starvation