Table 2.
Quantitative detection of C. botulinum in slug feces by qPCR
| Day | N positive | Mean Cq a (SD) | Mean log CFU/g (SD) |
|---|---|---|---|
| 2 | 3 (of 3) | 35.3 (±2.13) | 3.8 (±0.6) |
| 3 | 1b (of 3) | 38.5 (±1.01) | 2.9 (±0.7) |
| 4 | 1c (of 3) | 39.0 (±0.60) | 2.6 (±2.2) |
| 5 | 0 (of 3) | ND | ND |
| 8 | 0 (of 3) | ND | ND |
aMean Cq represents the mean of the positively quantified samples. Each sample was run in three qPCR replicates. The corresponding mean log CFU was calculated from the standard curve.
Additionally, 2b (or 1c) other samples were positive after TPGY-enrichment (Cq not shown).
qPCR, quantitative real-time PCR.
Cq, quantification cycle.
SD, Standard deviation.
CFU, Colony-forming units.
ND, Not detected; less than 2 replicates per sample yielded Cq values <40.