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. 2014 Oct 1;10:234. doi: 10.1186/s12917-014-0234-3

Table 1.

Procedures applied to embryos on breakout according to incubation period

Procedure Incubation day
0 - 2 3 - 15 15- 20
Weighing x x x
Candling x x x
Detection of embryo x x
Assessment of HR and VMM x x
Embryo euthanasia
  (<4°C, 4 h) x x
  (100%, CO2) x
Detection and measurement of blastodisc/blastoderm x
Detection and measurement of embryo x x
Weighing of embryo x x
Weighing of the albumen, yolk and shell x x x