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. 2014 Oct 7;16(1):85. doi: 10.1186/s12968-014-0085-x

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© Mikami et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Example of the expert Late Gadolinium Enhancement (LGE) segmentation procedure using a manual adjustment of the signal intensity (SI) threshold for each LGE image. A manual SI threshold (circled) was adjusted using a slide bar until visually identified LGE was segmented in accordance to expert opinion. Panel A shows a raw LGE image prior to application of segmentation. Panel B shows over-representation of LGE at a low SI threshold of 11. Panel C shows “optimal segmentation” at a threshold of 36. Panel D shows under-representation of LGE at a higher threshold of 78. The signal threshold of 36 (arrow) was applied for this image and the corresponding LGE area employed as a reference standard for semi-automated technique comparison.