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. 2014 Oct 2;12:274. doi: 10.1186/s12967-014-0274-2

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© Fu et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The ELISA assay for USCs-CM and the effect of USCs-CM on HUVECs. The concentration of VEGF (A) and TGF-β (B) in USCs-M and USCs-CM was measured by ELISA. (C) Real-Time Cell Analyzer showed USCs-CM significantly enhanced the migration of HUVECs. (D) CCK-8 assay showed USCs-CM significantly promoted HUVECs proliferation after 96 h incubation. (E and F) The scratch experiments demonstrated that USCs-CM increased the motility of HUVECs. (G) Large numbers of tube were formed in USCs-CM-cultured HUVECs, while no tube was formed in USCs-M-cultured HUVECs. *** P < 0.01 compared to the control or USCs-M groups at 16 h; *P < 0.05 compared to USCs-M groups. Scale bar = 250 μm.