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. 2014 Sep 26;15(1):816. doi: 10.1186/1471-2164-15-816

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© Santos et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Insert features of the BAC library. (A) Insert size distribution in a random sample of clones from the library (percentages on the y-axis); most of the clones had an insert size larger than 90 kb. (B) A typical agarose pulsed-field gel showing NotI digests of clones. The Lambda Ladder PFG Marker (New England Biolabs) is in the lanes at far left and right. Marker bands are given in kb, and the size of the vector (V) is 7,506 bp.