Figure 2.

Lack of 5-LO interferes on neutrophil migration. The neutrophils were gated based on their characteristic size (FSC) and granularity (SSC) (a). The dot plots represent the frequency of neutrophils population characterized by LY6G^highCD11b^high (upper gate) and LY6G^int⁡CD11b^int⁡ (lower gate) by flow cytometry (b). The bar graphs display the percentage and the absolute number characterized as LY6G^highCD11b^high population (c) or LY6G^int⁡CD11b^int⁡ (d) in the spleen from the WT and 5-LO^−/− mice at the 6th wpi or from uninfected mice (naïve mice). The data are expressed as the mean ± SEM, N = 5-6. *P < 0.05 compared with the WT naïve group, ^& P < 0.05 compared with the WT infected group, and ^# P < 0.05 compared with 5-LO^−/− naïve group.