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. 2014 Sep 21;2014:264270. doi: 10.1155/2014/264270

Figure 4.

Figure 4

The absence of 5-LO affected the cytokine release related to Th17 pattern. The spleen cells from the WT and 5-LO−/− mice at the 6th week pi or uninfected were in vitro stimulated with the L. infantum antigen (50 μg/mL) or medium for 72 hours, and the levels of IFN-γ (a), IL-17 (b), TNF (c), IL-23 (d), IL-6 (e), IL-1β (f), and TGF-β (g) were measured in the culture supernatants by ELISA assay. The data are expressed as the mean ± SEM and one representative of two independent experiments. *P < 0.05 compared to the medium; # P < 0.05 compared with the WT stimulation. The liver fragments from the WT (white bar) or 5-LO−/− (black bar) at the 6th wpi with the L. infantum promastigote forms were collected and weighed for the determination of IFN-γ (h), IL-17 (i), TNF (j), and IL-12p40 (k) by ELISA in the homogenate supernatants. The data are expressed as the mean ± SEM, N = 5-6. *P < 0.05 compared with the WT group.