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. 2014 Sep 21;2014:264270. doi: 10.1155/2014/264270

Figure 6.

Figure 6

The absence of 5-LO interferes with BMDC maturation and the release of innate cytokines induced by L. infantum through BLT1 receptor. The WT or 5-LO−/− BMDC was stimulated with L. infantum (5 : 1) (black bars), LPS (200 ng/mL) (hatched bars), or medium (white bars) for 24 h. The BMDCs were harvested and the costimulatory molecules expression such CD86 (a), MHC-II (b), and CD40 (c) was evaluated by flow cytometry. All analyses were performed for the CD11chigh population. The TNF (d), IL-23 (e), IL-1β (f), and IL-6 (g) levels were measured in the supernatant of the BMDC culture by ELISA assay. The data are expressed as the mean ± SEM and are representative of three independent experiments. *P < 0.05 compared with the medium; # P < 0.05 compared with the infected WT. & P < 0.05 compared with the infected WT. In some experiment, selective BLT1 leukotriene B4 receptor antagonist (10 μM) was or not added 12 h before L. infantum infection or LPS stimuli. The levels of TNF (h), IL-23 (i), and IL-6 (j) were determinate into supernatant 24 thereafter. *P < 0.05 compared with the medium; # P < 0.05 compared with L. infantum infection. & P < 0.05 compared with LPS stimuli.