Figure 1.

Supernatant of HeLa, SiHa, and C-33A induces CD163 upregulation in U937-derived macrophages activated with lipopolysaccharides (LPS). U937 cells were differentiated into macrophages and treated with LPS for 24 h. Supernatants of cervical cancer (CC) cells were added to a final concentration of 30% in corresponding groups, and the cells were incubated for 7 days at 37°C in a humid atmosphere containing 5% CO₂ and 95% air in Roswell Park Memorial Institute- (RPMI-) S medium. Then, the expression of CD163 was analyzed by flow cytometry (FC). Expression of CD163 in U937-derived macrophages activated with LPS and treated with supernatant of HeLa, SiHa, and C-33A cells during 3, 5, and 7 days (a). Percentage of CD163 expression in U937-derived macrophages activated with LPS and treated with supernatant of HeLa, SiHa, and C-33A during 7 days (b). Values of geometric mean fluorescence intensity (MFI) for CD163 expression in the different experimental groups (c). Dexamethasone (DEX) was used as positive control in the induction of CD163 expression. Results are shown as % and MFI and represent the mean ± standard deviation (SD) of three independent experiments performed in triplicate. Statistical analysis, Student's t-test. ^◆ P < 0.05 LPS versus Basal group. ^• P < 0.05 HeLa, SiHa, and C-33A groups versus Basal and LPS groups. *P < 0.0001 DEX versus all groups.