Figure 4.

Supernatant of cervical cancer (CC) cells induces a decrease in Nitric oxide (NO) in U937-derived macrophages activated with Lipopolysaccharides (LPS). U937-derived macrophages were activated with LPS and treated or not with supernatant of HeLa, SiHa, and C-33A cells or Dexamethasone (DEX) for 7 days. After culture, supernatant was collected. Quantification of NO in the form of Nitrate (NO₃ ⁻) was determined in the supernatant of all experimental groups by colorimetric assay. The results represent average concentrations in μmol/L. Values represent mean ± standard deviations (SD). Statistical analysis, Student's  t-test. ^• P < 0.05 LPS and Basal versus HeLa, SiHa, and C-33A groups.