. 2014 Sep 25;10:223. doi: 10.1186/s12917-014-0223-6

Table 2.

Primers used in this study

Primers	Sequence (5′ → 3′)	Localization (nt) ^c	Size (bp)
eno1F	CGAGAGCTC ^aATGTCAGCAATTAAAAAAATCC	1	202
eno1R	CACCTTTTCCACCAAAC ^bCAATTAG	202	202
eno2F	CTAATTGG ^bTTTGGTGGAAAAGGTG	179	785
eno2R	AAATCCAGCC ^bCAGTCGCTTT	963	785
eno3F	AAAGCGACTGG ^bGCTGGATTT	944	399
eno3R	CGGCTCGAG ^aTTATTTTTTAAGATTGTA	1342	399

^aThe restriction sites of GAGCTC, CTCGAG for endonuclease SacI and XhoI were shown in italics.

^bNucleotide substitutions were shown in boldface.

^cLocalization was based on the nucleotide sequence of M. synoviae 53 eno gene (GeneID:3564671).