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. Author manuscript; available in PMC: 2014 Oct 8.
Published in final edited form as: Methods Enzymol. 2014;542:349–367. doi: 10.1016/B978-0-12-416618-9.00018-2

Figure 4. Subcellular distribution of NADH in mammalian cells.

Figure 4

(A) Fluorescence intensities with excitation at 410 nm or 500 nm normalized to the initial value; emission at 528 nm. (B) Ratiometric fluorescence images of cells expressing Frex in mitochondria, the nucleus, and cytosol. The pixel-by-pixel ratios of the 488 nm excitation image by the 405 nm excitation image of the same cell were used to gray the images. Scale bar, 10 μm.