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. Author manuscript; available in PMC: 2014 Oct 8.
Published in final edited form as: Parasitology. 2013 May 23;140(9):1104–1110. doi: 10.1017/S0031182013000504

Fig. 1.

Fig. 1

PL2-6 antibody reactivity with Toxoplasma gondii histones. (A) T. gondii recombinant histones H2A.1 (canonical), H2AX, H2AZ, H2Ba (canonical) and H2Bv were assayed by Western blot (WB) with PL2-6 antibody. Recombinant HSP28 was included as non-histone control. The upper panel shows the Ponceau red staining as loading control. The lower panel shows the WB using mAb PL2·6 antibody. Molecular weight markers are shown in both marginal lanes; (B) The nuclear fraction of T. gondii extracellular tachyzoites was resolved in 15% SDS-PAGE. Lane one was Coomassie-blue stained to detect H2A, H2B, H3 and H4 histones. The other lane were cut into pieces and assayed by WB with PL2-6, anti-H2Bv, anti-H2AX, anti-H3 and anti-H4 trimethylated K20 (H4) antibodies.