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Identification of epichromatin in Toxoplasma gondii tachyzoites. Extracellular T. gondii tachyzoites were fixed either with paraformaldehyde (PAF) or methanol (see Materials and Methods) and assayed by immunofluorescence assay (IFA) using PL2-6 antibody and DAPI to stain the nuclear DNA. Epichromatin epitope identified with murine mAb antibody (PL2-6) was stained with Alexa Fluor 488 (green). PH, phase contrast.
