Table 1. Summary of Global Proteomic Analyses of ETI-Regulated Proteins.
| T3SE | NB-LRR-protein | Cellular Fraction/PTM enrichment |
Proteomics Approach | Protein IDsa | Reference | |
|---|---|---|---|---|---|---|
| AvrRpm1 | RPM1 | Chloroplast,Mitochondria,Cytosol | 2D-PAGE | GSTF8 (At2g47730) GST79 (At2g30860) OEC33 (At5g66570) OEC23 (At1g06680) |
17 , 18 | |
| AvrRpm1 | RPM1 | Secreted Proteins | SDS-PAGE iTRAQ LC-MS/MS |
Ubiquitin GAPDH(At1g13440/At3g04120) Phosphoglucomutase (At1g23190) Enolase (At2g36530) |
19 | |
| AvrPto/HopAB2 | Prf | All soluble proteins | iTRAQ 2D-LC-MS/MS |
14–3-3 protein (TC217464_3, TC217870_1, TC233482_2) Acyl-binding carrier protein (TC238115_2) Acyl-carrier protein transacylase (TC219533_3) |
20 | |
| GVG:AvrRpm1 | RPM1 | Microsome, Cytosol | 2D-PAGE MALDI-TOF |
AtRem1.2 (At3g61260) PP2C PIA1 (At2g20630) C2-domain containing protein (At4g34150) |
44 , 45 | |
| GVG:AvrRpt2 | RPS2 | Plasma Membrane | SDS-PAGE LC-MS/MS Label free quantitation |
SOBIR1 (AT2G31880) RIPK (AT2G05940) PIA1 (At2g20630) SYP122 (AT3G52400) SOBER1(At4g22300) PLDγ1 (AT4G11850) DGK5 (AT2G20900) |
PEPR1 (AT1G73080) WAK1 (AT1G21250) BIK1 (AT2G39660) SNAP33 (AT5G61210) PEN1/SYP121 (AT3G11820) PLDα1 (AT3G15730) |
49 |
| AvrRpm1 | RPM1 | Phosphorylated Proteins | iTRAQ LC-MS/MS |
Rubsico large subunit (AtCg00490) | 60 | |
| AvrB | RPM1 | S-nitrosylated cysteine | Biotin-Switch SDS-PAGE MALDI-TOF/TOF | Rubsico large subunit (AtCg00490) GAPDH (At3g26650) Triosephosphate isomerase (At3g55440) PsbQ (At4g21280) |
65 | |
| AvrB | RPM1 | Tyrosine nitration | Immunoaffinity enrichment 2D-PAGE LC-MS/MS |
Rubsico large subunit (AtCg00490) PsbO2 (At3g50820) PsbO1 (At5g66570) Fructose-bisphosphate aldolase 1 (At2g39730) Fructose-bisphosphate aldolase 2 (At4g38970) |
66 | |
Note: aTomato gene indices as annotated by DFCI – LeGI