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. 2014 Jun 6;23(21):5638–5648. doi: 10.1093/hmg/ddu280

Figure 3.

Figure 3.

Generation of SPG3A iPSC lines. (A) Control and SPG3A iPSC lines expressed the human ESC markers Tra-1-60 (red), Nanog (red) and SSEA-4 (green), as visualized by immunofluorescence microscopy. Hoechst nuclear staining is blue. Scale bars: 100 µm. (B) SPG3A iPSC lines maintained a normal 46,XX female karyotype after 10 passages as shown by G-banded analysis. (C) DNA sequencing electropherograms confirm the presence of a heterozygous c.1024C>T, p.Pro342Ser mutation before and after neural differentiation.