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. 2014 Jun 6;23(21):5683–5705. doi: 10.1093/hmg/ddu285

Figure 9.

Figure 9.

Behavioral characterization of the humanized DISC1-Boymaw mice on 129S background. (A) Breeding of wild-type and the heterozygous DISC1-Boymaw mice on 129S pure genetic background. 129S mice carry a frame-shift mutation in exon 6 of the disc1 gene. (B) The cohort consisted of 14 wild-type females, 14 heterozygous females, 13 wild-type males and 15 heterozygous males. The age range of the birth date between individual mice was less than a week. Body weight was compared between the wild-type and the heterozygous mice. Heterozygous males displayed slightly, but significantly, reduced body weight at postnatal Day 103 [unpaired, two-tailed student's t-test, t(26) = 2.132, P < 0.05]. No difference was found in body weight between female wild-type and heterozygous mice. (C) There was a significant gene effect [F(1,54) = 4.41, P < 0.05] in PPI at 69 dB prepulse. A significant gene X ISI interaction was also observed [F(4,216) = 2.90, P < 0.05]. Post hoc analysis revealed that the heterozygous DISC1-Boymaw mice displayed increased PPI (or impaired prepulse facilitation) with ISI at 25 ms. No sex difference was observed. (D) The male heterozygous DISC1-Boymaw mice display a significant gene effect [F(1,26) = 6.26, P < 0.05] as well as a gene X ketamine interaction [F(1,26) = 8.61, P < 0.01]. Post hoc analyses (Tukey studentized range test) revealed that the male heterozygous mice displayed significantly increased and prolonged responses to ketamine in comparison with wild-type male controls in all time blocks except the first and last one. (E) Total immobility time (seconds) was recorded during a 6 min suspension test. Five wild-type male mice were lost because of fighting in cages after previous tests. The heterozygous DISC1-Boymaw mice (n = 29) displayed significantly more immobility time than the wild-type control mice (n = 22) [unpaired, two-tailed student's t-test, t(49) = 2.22, P < 0.05). (F) Two or three mice were grouped in a single cage according to genotype. Saccharin preference test was conducted for four consecutive days. Consumption of saccharin was measured each day, and compared between the wild-type and the heterozygous mice in each sex. Significant gene effect was detected [F(1,11) = 17.85, P < 0.01] between female wild-type (n = 7) and the DISC1-Boymaw heterozygous mouse cages (n = 6). Post hoc analysis revealed that the female heterozygous mice displayed significantly less saccharin consumption than female wild-type controls mice at Day 2 and Day 4. Error bar: SEM. #P < 0.10; *P < 0.05; **P < 0.01.