Table 4. Similarity of barnacle adhesive cDNA and AA sequences between different species.

	cp-19k AA	cp-19k DNA	cp-20k AA	cp-20k DNA	cp-100k AA	cp-100k DNA
A. improvisus, F. albicostatus	60%	49%
A. improvisus, F. albicostatus, M. rosa	42–44%	36–40%
A. improvisus, F. albicostatus, P. Pollicipes, M. rosa		26–36%
F. albicostatus, A. amphitrite			34–46%	29–36%	64%	49%
F. albicostatus, A. amphitrite, M. rosa			18–21%	19–30%	42–45%	37–40%
F. albicostatus, A. amphitrite, P. pollicipes, M. rosa						26–35%

Similarity was calculated using GeneDoc [41], based on Clustal W alignments created in Mega5 [40]. Similarity is calculated for species pairs, so for groups of more than two species a range of similarity is given. Two variants of cp-20k exist in A. amphitrite and both were included in alignments, resulting in a range of similarity values for the cp-20k two species comparison.