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. 2014 Oct 8;9(10):e109201. doi: 10.1371/journal.pone.0109201

Figure 1. Characterization of the transposon mutant library of violacein producing clone 20G8.

Figure 1

(A) Several mutants exhibit changes in violet pigmentation typical of the presence of violacein: 20G8vioA − and 20G8vioB − mutants have lost pigmentation (white arrows). 20G8vioC− and 20G8vioD− mutants express green-grey pigmentation (black arrows). (B) Nematode killing assay (N2 animals vs bacterial strains 20G8 clone and 20G8vioABCD − mutants). Survival kinetics of nematodes fed with either the 20G8 clone or the 20G8vioABCD − mutants deficient in violacein production. The killing phenotype of the four mutant clones is significantly reduced (p<0.0001) when compared to the wild type clone 20G8. A randomly chosen clone from the library with no activity is used as a negative control. Each data point represents means ± the standard error of three replicate plates. p values were calculated on the pooled data of all of the plates in each experiment by using the log-rank (Mantel–Cox) method.