Figure 6. Nematode killing assay (wild type animals and daf-2, daf-2;spp-1, daf-2;lys-7, daf-2;sod-3, spp-1, lys-7, sod-3 mutant nematodes vs the 20G8 clone).

(A) The survival of the nematode was tested using C. elegans double mutants daf-2;sod-3, daf-2;spp-1, daf-2;lys-7 and (B) the single mutant animals sod-3, spp-1, and lys-7. Each data point represents means ± the standard error of three replicate plates. p values were calculated on the pooled data of all of the plates done in each experiment by using the log-rank (Mantel–Cox) method and the values are provide in the text.