. 2014 Oct 8;9(10):e109201. doi: 10.1371/journal.pone.0109201

Table 1. Bacterial strains and vectors used in this study.

Strain/Vector	Relevant characteristic or genotype	Source or reference
E. coli EPI300-T1^R	F-mcrA Δ(mrrhsdRMSmcrBC) φ80dlacZΔM15ΔlacX 74 recA1 endA1 araD139 Δ(ara, leu) 7697galU galK λ- rpsL nupG trfA tonA dhfr	Epicentre
E. coli 20G8	Fosmid 20G8 cloned in EPI300-T1^R; Cm^r	This study
E. coli 20G8vioA^-	Fosmid 20G8 mutated in vioA gene and cloned in EPI300-T1^R; Cm^r, Kan^r	This study
E. coli 20G8vioB^-	Fosmid 20G8 mutated in vioB gene and cloned in EPI300-T1^R; Cm^r, Kan^r	This study
E. coli 20G8vioC^-	Fosmid 20G8 mutated in vioC gene and cloned in EPI300-T1^R; Cm^r, Kan^r	This study
E. coli 20G8vioD ^-	Fosmid 20G8 mutated in vioD gene and cloned in EPI300-T1^R; Cm^r, Kan^r	This study
E. coli OP50	Uracil auxotroph	[29]
Microbulbifer sp. D250	Wild type strain	[62]
Microbulbifer sp. D250 dv2	D250 strain mutated in vioB gene; Kan^r	This study
pLof/Tn10 KM	Mini-Tn10 (Kan^r); Amp^r	[34]
pCC1FOS^a	Fosmid backbone for genomic library; Cm^r	Epicentre

Copy number inducible by arabinose.