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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Dev Biol. 2014 Aug 28;395(1):144–153. doi: 10.1016/j.ydbio.2014.08.015

Figure 2. Fluorescence activated cell sorting of Kaede positive cells from head tissues dissociated from 24hpf Tg[flhBAC:kaede] vu376 embryos.

Figure 2

(A) Cells first undergo forward scatter (FSC-A) and side scatter (SSC-A) analysis to be separated based on cell size and internal granularity, respectively. Only the cells from the selected colored region are subsequently sorted for viability. (B) Viable cells do not take up propidium iodine (PI-A). (C) Intact, viable cells then get sorted based on fluorescent intensity.