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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Dev Biol. 2014 Aug 28;395(1):144–153. doi: 10.1016/j.ydbio.2014.08.015

Figure 6. Differentially expressed genes identified from RNA sequencing experiments were validated using in situ hybridization.

Figure 6

At 24 hpf, expression of otx5, rdh5, gngt2a, rgra, gngt1, and otomp, shown to be downregulated in tbx2b morphant pineal transcriptome as compared to controls by 2.2, 3.1, 3.0, 3.7, 6.4, and 7.8 fold, respectively, are reduced in tbx2b morphant (A) and tbx2bc144−/− (B) pineal complex as compared to NIC or WT. tbx2b and oep, display no significant change in FPKM values from RNA seq experiments (C), and are used as controls. (A and B) Dorsal view of the epithalamus at 24 hpf. Scale bar: 30 μM