Table 1.

Templates and enzymes used to synthesize antisense RNA probe

Plasmid name	Reference	Enzyme used to linearized plasmid	RNA polymerase
pBS-SK-oep5A1	Zhang et al., 1998	HindIII	T3
pCR4-TOPO-otomp/zomp-1	Murayama et al., 2005	NotI	T3
pBS-otx5	Gamse et al., 2002	EcoRI	T7
pCRII-TOPO-rdh5	Nadauld et al., 2006	EcoRV	Sp6
pME18S-FL3-rgra	Open Biosystems (Clone ID 8123753)	PCR amplified. Forward primer: 5′ TGCTCTAAAAGCTGCGGAAT 3′ Reverse primer: 5′ TAATACGACTCACTATAGGGGGGAGGTGTGGGAGGTTTT 3′	T7
pCRII-tbx2b	Snelson et al., 2008b	BamHI	T7