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In situ tapping-mode AFM brush height determined over a relatively narrow pH window for micropatterned PTBAEMA brushes immersed in aqueous solution: (■) linear PTBAEMA brush (original dry brush thickness = 13 nm); (●) uniformly cross-linked PTBAEMA brush prepared using PPG-TDI in THF; (⧫) surface-cross-linked PTBAEMA brush prepared using PPG-TDI in n-hexane.
