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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 Aug 31;21(10):864–870. doi: 10.1038/nsmb.2880

Figure 5.

Figure 5

Sgo1 prevents Wapl from accessing a functional site on cohesin. (a) Quantification of the percentages of mitotic GFP-sororin 9A-expressing HeLa cells (transfected with the indicated siRNA and arrested in prometaphase with nocodazole or at metaphase with MG132) that had unseparated chromatids (types I/II) and separated chromatids (types III/IV). Error bars, range (n = 2 independent experiments). (b) Quantification of the mitotic indices (defined as the percentage of cells with 4N DNA content and positive MPM2 staining) of HeLa cells transfected with Sgo1 siRNA and the indicated Myc-SA2 plasmids. WT, wild type. Error bars, range (n = 2 independent experiments). The K901E and K949E E950K mutants were tested only once. (c) Model for sororin- and Sgo1-dependent cohesion protection during the cell cycle. Sgo1 protects centromeric cohesion in two ways: enabling sororin dephosphorylation by PP2A and directly shielding SA2–Scc1 from Wapl. Ac, acetyl group.