Table 3. List of novel chickpea miRNAs with their miRNA*.

New miRID	Sequence	L	miRNA*	Start	End	PL	MFE	Adjusted	Hairpin
								MFE	G/C%
car-miRNA001	AACCAGGCUCUGAUACCAUGA	21	AUGGUAUCAGGUCCUGCUUCA	20306919	20306939	87	−37.34	−42.91954	42.528736
car-miRNA002	AAGAUUGAUCUUGACCUUCUGC	22	UUAUGGCAUAAACAAGGAUAAU	588	609	93	−29	−31.182796	37.634407
car-miRNA003	AAGCAGGCUCUGAUACCAUGA	21	UGGUAUCAGGUCCUGCUUCA	696	716	95	−42.3	−44.526314	47.368423
car-miRNA004	AAUAGAUUGUCCAAUCGAUUGU	22	CAAUCGAUUUCCCAAUCGAUUU	343	364	159	−47.8	−30.062893	33.962265
car-miRNA005	AAUCACGGUGAGCCACUGUGA	21	AAUCACGGUGGCUCACCGUGA	251	271	91	−54.2	−59.56044	39.56044
car-miRNA006	ACCGGAAGCUGGGUUACGGUC	21	CGCGACCUAUACCCGGCCGU	598	618	199	−75.01	−37.693466	56.281406
car-miRNA007	ACGACUGUUACAUCAUACAAC	21	UGUAUGGUGCAACAGUCGCAG	23710719	23710739	137	−65.8	−48.029198	43.065693
car-miRNA008	ACGAGACAGAUGGACACGACGG	22	CGUACGUUGUCGGAUAUGUCGC	338	359	97	−28.5	−29.381443	47.42268
car-miRNA009	AGCGAUCUCGUACUAAACGAA	21	CUUCGAUAGGCGAGAGGUGUA	23987481	23987501	78	−24.9	−31.923077	47.435898
car-miRNA010	AGGAGAAAGUCUUUGCAACCG	21	UGUGUUGCUGAGACAUGCGCC	273099	273119	65	−21.7	−33.384617	52.307693
car-miRNA011	AUGGUUGAGAGGGUGACUUGA	21	AAGUCACUUUCUCAAUCUUA	1161	1181	155	−72.3	−46.645164	39.35484
car-miRNA012	CAGGGAACAGGCUGAGCAUGG	21	AUGCACUGCCUCUUCCCUGGC	171	191	87	−47.7	−54.827587	51.724136
car-miRNA013	CAGGGAACAGGCUGAGCAUGG	21	AUGCACUGCCUCUUCCCUGGC	149	169	87	−47.7	−54.827587	51.724136
car-miRNA014	CAGGGAACAGGCUGAGCAUGG	21	AUGCACUGCCUCUUCCCUGGC	285	305	87	−47.7	−54.827587	51.724136
car-miRNA015	CGAGACAGAUGGACACGACGG	21	CGUACGUUGUCGGAUAUGUCG	336	356	97	−28.5	−29.381443	46.391754
car-miRNA016	CGAUUGCGGCGACGUGGGCG	20	CUGCCCGCGACGUUGUGAGA	50	69	66	−32.8	−49.696968	57.575756
car-miRNA017	CGGAAUACAAGCUCUGUACCGGAA	24	CGGAACACUCUUCUGUACCGGAAA	18012181	18012204	116	−42.41	−36.560345	39.655174
car-miRNA018	CUGACUUAGCUUGUAGUCGAC	21	UAGUCGACUACAGAUGGGUGU	533	553	67	−30.5	−45.52239	46.268658
car-miRNA019	CUGGGUUGGGUCGAUCGGUCC	21	CACCGGUUGGCUCGUCCCUU	842	862	75	−32.8	−43.73333	65.33333
car-miRNA020	CUGUAGCAUCACUAUAGCCGC	21	CGGCUAUAGUGGCGCUAUAGC	37423709	37423729	95	−45	−47.368423	42.105263
car-miRNA021	GAAACGGGUAGCUGAGGGUU	20	CACUCUAAACAGCAGCUCCGU	580	599	113	−34.3	−30.353981	40.707962
car-miRNA022	GAAACGGGUAGCUGAGGGUU	20	CACUCUAAACAGCAGCUCCGU	499	518	113	−32.3	−28.584068	39.823006
car-miRNA023	GAAAUGGACGGCAAUGAAUCUA	22	UUGAAGGUUUUGCUGACCUUU	157	178	88	−26.52	−30.136364	42.045452
car-miRNA024	GAACGAGACAGAUGGACACGA	21	UACGUUGUCGGAUAUGUCGCGA	337	357	98	−29.9	−30.510204	45.918365
car-miRNA025	GAGUUCACUGUUGGAGAUGUGCCA	24	GCACAACUCCAACGGUGAACCCAC	30794763	30794786	220	−138.6	−63.000004	45.909092
car-miRNA026	GCCGGCCUGUCAGACCUAAUAGGC	24	UCAAGCCAUAGGCCUCUGACGGAC	9008149	9008172	162	−62.4	−38.518517	41.975307
car-miRNA027	GCGAAGCUAUCGUGCGUUGGAU	22	UUCGCACAAUUGGUCAUCGCG	138313	138334	95	−32.3	−34	50.526314
car-miRNA028	GGGUUGGGUCGAUCGGUCCA	20	CACCGGUUGGCUCGUCCCUU	677	696	69	−33.8	−48.985504	63.76812
car-miRNA029	GGGUUGGGUCGAUCGGUCCGCC	22	GGUGUGCACCGGUUGGCUCGU	18682982	18683003	69	−35.5	−51.449276	66.66667
car-miRNA030	GGGUUGGGUCGAUCUGUCCGCC	22	GGUGUGCACCGGUUGGCUCGU	619	640	69	−29.3	−42.463768	65.21739
car-miRNA031	GGUUGGGUCGAUCGGUUCGCCU	22	GUGUGCACCGGUCUGCUCGUC	383	404	69	−30.9	−44.782608	65.21739
car-miRNA032	GUUCUAGAUCGACGGUGGCAU	21	GUCACCACCGUCGUCUCGCA	103	123	66	−28.6	−43.333332	56.060608
car-miRNA033	GUUCUAGAUCGACGGUGGCAU	21	GUCACCACCGUCGUCUCGCA	111	131	66	−28.6	−43.333332	56.060608
car-miRNA034	GUUCUAGAUCGACGGUGGCAU	21	CACCACCGUCGUCUCGCAGCU	313	333	67	−27.6	−41.19403	56.71642
car-miRNA035	UAACUCUGAUGAAGUUGUGCA	21	GCUCAAUUUGUAUCUGGGACAU	147	167	61	−19.3	−31.639343	37.704918
car-miRNA036	UAAGUCGGUGACGUCUACGUAUAC	24	UCUAUACGGAAGAUGCAUGGACUA	21957421	21957444	82	−24.2	−29.512197	41.463413
car-miRNA037	UAGCGACACGGAACGUCCAAC	21	GGGGUUGGACGCUCCGUGCCA	215357	215377	112	−81.4	−72.67857	51.785713
car-miRNA038	UAUGUGAACGAGACAGAUGGA	21	UCGGAUAUGUCGCGACACAAA	342	362	98	−29.9	−30.510204	45.918365
car-miRNA039	UCAUAUUUGUUGGACAUUUGA	21	UCAAUGUAUUGAUGGGUAUGU	942	962	113	−29.7	−26.283186	38.938053
car-miRNA040	UCGCGUGAGUGAAGAAGGGCA	21	AGCUCUUUCGUCGAGUGCGCG	69427	69447	66	−23.3	−35.30303	50
car-miRNA041	UCGCUGUUGCGUUGGCGAUUA	21	AUCGCCACCGCAACAGCGAAG	21404687	21404707	114	−59.83	−52.482456	53.50877
car-miRNA042	UCGGACCAGGCUUCAUUCUUC	21	AAUGAGGUUUGAUCCAAGAUC	1317	1337	197	−57.8	−29.3401	38.071068
car-miRNA043	UGAACUAUUCGAUCUUCGUUC	21	GAUGAAGAUCAAACGGUUCAU	101	121	147	−67.8	−46.122448	32.65306
car-miRNA044	UGAAGCUGCCAGCAUGAUCUUA	22	AGAUCAUGUGGCAGUUUCACC	1047	1068	73	−36.4	−49.863018	47.945206
car-miRNA045	UGAUUGUAUAAUCGAUUAGGCA	22	AUUGGCAAAUCGAUUGUGCACA	26	47	121	−47.8	−39.50413	38.842976
car-miRNA046	UGAUUGUAUAAUCGAUUAGGCA	22	AUUGGCAAAUCGAUUGUGCACA	26	47	121	−47.8	−39.50413	38.842976
car-miRNA047	UGCCAAGCGCUGUAGUAGGUCA	22	AUAAGGCUUUUACAGCGCUUG	606	627	94	−50.1	−53.29787	44.68085
car-miRNA048	UGGACUAAAAUUCUGUUUGGAGAC	24	GUCCCCCAAGCAGAAUUUUGGUCC	30591970	30591993	218	−91.99	−42.197247	31.192661
car-miRNA049	UGGACUAAAAUUCUGUUUGGAGAC	24	GUCCCCCAAGCAGAAUUUUGGUCC	47072	47095	220	−95.82	−43.554543	31.818182
car-miRNA050	UGGAUUGAGAUCGAAUGGUGC	21	GACCGUCCGGUCUUGAUCCAAG	347	367	137	−60.2	−43.941605	37.956203
car-miRNA051	UGGGACAAUCGAUUUGGACAUC	22	UUGGAAAUCGAUUGAUUCAGUG	260	281	72	−22.4	−31.111109	34.72222
car-miRNA052	UGGUCUGUGAGAGACUGCACGGUA	24	UCGUGCUGGUCUGUGAGAGACUGC	52521	52544	96	−40.7	−42.395832	53.125
car-miRNA053	UGGUUGGGUCGAUCGGUCCGCC	22	GGUGUGCACCGGUUGGCUCGU	677	698	85	−37.1	−43.647057	63.529415
car-miRNA054	UGUGGAUGAUGCAGGAGCUGA	21	AGCUGCUGACUCAUUCAUUCA	27572322	27572342	152	−59.5	−39.144737	35.526314
car-miRNA055	UGUUGCAAUCGACCAGGACUAC	22	AGUCUUGAUCGAUGUAACUGA	385	406	79	−39.5	−50	32.911392
car-miRNA056	UUAACCAGGCUCUGAUACCAU	21	UGGUAUCAGGUCCUGCUUCA	21161303	21161323	88	−37.4	−42.5	50
car-miRNA057	UUCGAAUCCUGCCGUCCACGCC	22	AGUGGACGUGCCGGAGUGGUUA	29887519	29887540	94	−37.2	−39.574467	55.31915
car-miRNA058	UUGAGCCGCGUCAAUAUCUUG	21	CAAGGUAUUGGCGCGCCUCAA	24546067	24546087	92	−53.4	−58.04348	41.304348
car-miRNA059	UUGAUCUUUCGAUGUCGGCU	20	GUUUAGACCGUCGUGAGACA	988	1007	116	−45.4	−39.13793	50.86207

MFE: Minimum Folding Energy; L: Length; PL: Precursor Length.