Figure 2. Estimation of divergence times of non-recombinant HBV subgenotypes D1–D7 including Indian HBV/D4 (IndD4).

The tree was constructed by the DNAMLK program of the PHYLIP package, using indel-free HBV sequences of different subgenotypes of D, derived from GenBank, along with 12 IndD4 sequences. Reference sequences are indicated by their specific subgenotypes followed by accession numbers and the IndD4 sequences are indicated by respective isolate numbers beginning with “TP.”