Table 1. Primers used in this study.
Primer Name | Primer sequence | Location [nt**] |
F1 | 5′-CACAAGAGGACTCTTGGACT-3′ | 1653–1672 |
F3 | 5′-CGCCTCATTTTGTGGGTCAC-3′ | 2801–2820 |
F4 | 5′-CTCAGGCCATGCAGTGGAA-3′ | 3164–3182 |
F5 | 5′-GATGTGTCTGCGGCGTTTTA-3′ | 376–395 |
F7 | 5′ -TGTGCACTTCGCTTCACCTC -3′ | 1578–1597 |
F8 | 5′ -ATTTGCATACTCTTTGGAAGGC-3′ | 2746–2767 |
F10 | 5′ -GACCACCAAATGCCCCTATC -3′ | 2298–2317 |
R1 | 5′-CCACCTTATGAGTCCAAGG-3′ | 2457–2475 |
R2 | 5′-AAATTACCACCCACCCAGG-3′ | 2109–2127 |
R3 | 5′-AACTGGAGCCACCAGCAG-3′ | 57–74 |
R5 | 5′-AAAGCCCAAAAGACCCACAAT-3′ | 996–1016 |
R8 | 5'-CTTTGACAAACTTTCCAATCAAT- 3' | 973–995 |
R9 | 5'-TAGGAGTTCCGCAGTATGGA- 3 | 1265–1284 |
SP2 | 5′-GTATGGTGAGGTGAACAATG-3′ | 2039–2058 |
MP1 | 5′-GAGCTCTTCTTTTTCACCTCTGCCTAATCA-3′ | 1821–1841 |
MP2 | 5′-GAGCTCTTCAAAAAGTTGCATGGTGCTGG-3′ | 1806–1825 |
P1ψ | 5' -CCGGAAAGCTTGAGCTCTTCTTTTTCACCTCTGCCTAATCA- 3′ | 1821–1841 |
P2ψ | 5'-CGGAAAGCTTGAGCTCTTCAAAAAGTTGCATGGTGCTGG- 3′ | 1823–1806 |
** = nucleotide [nt] positions are given according to HBV sequence with accession no. AF121242 obtained from GenBank.
Gunther S et al. J. Virol. 1995; 69: 5437–44.