Table 3. Conserved amino acid residues in four open reading frames of Indian HBV/D4 (IndD4) isolates compared with the previously reported residues at the same position in different subgenotypes of D (D1–D9).

Amino acid position	Amino acids in subgenotypes of HBV/D
	D1	D2	D3	D4	D5	D6	D7	D8	D9	IndD4 [n = 12]
PreS/S gene
PreS1-27*	T	T/S	T	T	T	T	T	H	T	S
PreS2-54	L	L	L/P	L/R	P/L	Q/L	L	S	L/P	Q
s120**	P	P	P	P	P	P	P	P	P	T
s129	Q/R	Q	Q	Q	Q/H	Q	Q/H	Q	Q	R
PreC/C gene
c159	P	P	P/Q/T	P	P	P/Q	P	P	T/A/P	T
X-Gene
x11	P	P	P	P	P	P	P	P	P	L
x12	A	A/S	A	A	A	A	A/T	A	A	T
Polymerase Gene a
tp87	D	D	D	D	D	D	D	D	D	N
tp90	K	K	K	K	N/Y	K	K	K	K/N	E
sp30*	H	H/L	H	H	H	Y	H	P	H	F
sp62	T	T	T	T	T	T	T	S	T	A
sp66	P/S	P	P	P	P	P	P	R	P	S
rt122	F/V	F/V	L	I	F	L	F	F/H	L/I/V	F
rt128**	T	T	T	T	T	T	T	T	T	N
rt267	Q	Q	Q	H/Y	H	Q	H	H	Q/H	L
rt333	K	K	K	K	K	K	K	K	K	Q
rh50	S	S	S	S	S	S	S	S	S	F
rh54	I/L	I	I	I	I/V	I	I/V	I	I/L	V
rh139	R	R	R	R	R/H	R	R	R	R	H

Unique residues of IndD4 are underlined. The major amino acids are indicated in bold. ^aThe conserved domains of the open reading frame of HBV polymerase are indicated as follows: tp, terminal protein domain; sp, spacer region; rt, reverse transcriptase domain; rh, RNase H region. *, ** the overlapping surface and polymerase mutations