Table 2.

Analyzed polymorphisms within AREG and EREG

Gene SNP	Allele	MAF	Function	Forward (F) and Reverse (R) primer
AREG rs161511	G>A	7%	splicing regulation	F: 5′-TCTCCACTCGCTCTTCCAAC-3′ R: 5′-CCTCACCTGAGCCGAGTATC-3′
AREG rs28364983	T>C	23%	transcriptional regulation	F: 5′-ATGGCTGTTACCCCT GTGAG-3′ R: 5′-GGGGGCTTAACTACCTGTTCA-3′
AREG rs1691263	C>T	25%	unknown	F: 5′-AAAAAGGGAGGCAAAAATGG-3′ R: 5′-CCTGCAGTTAGGGGTTTCAG-3′
AREG rs2291715	A>C	32%	splicing regulation, transcriptional regulation	F: 5′-AAAACACACCGCACGTTTTT-3′ R: 5′-AACAGCAACAGCTGTGAGGA-3′
EREG rs10446508	A>T	36%	transcriptional regulation	F: 5′-CCCCCAAAACTCTTCTACCTTT-3′ R: 5′-CAGCCCATGGAAATATAGGG-3′
EREG rs1962685	C>T	25%	transcriptional regulation	F: 5′-CTCCCAAACTGCTGGGATTA-3′ R: 5′-GGGCACAGATGTTCAAGTCA-3′
EREG rs2061509	C>T	31%	transcriptional regulation	F: 5′-TCCTTAGAAGGGAGGAAGGAA-3′ R: 5′-GGGATGAAGAGGTGATGTGC-3′
EREG rs7687621	C>T	17%	transcriptional regulation	F: 5′-TGATGTGGTTTCCTCAAAGC-3′ R: 5′-ATAATGTGCCCAAGGTCCAA-3′
EREG rs12641042	C>A	46%	transcriptional regulation	F: 5′-TCCAGTCAGAAACCATGCAA-3′ R: 5′-CACAGTGCAAGTCTGCTGGTA-3′
EREG rs7575690	T>C	15%	transcriptional regulation	F: 5′-CTGCATGATTTCCAGGATTG-3′ R: 5′-TGCCTACCTCCAAATTCAACA-3′

AREG = amphiregulin; EREG = epiregulin, MAF = minor allele frequency (Japanese ethnicity), T= thiamine, C= cysteine, A= adenine, G= guanine