Primary rat OL cultures were subjected to normoxia or OGD in the presence or absence of vehicle, 200 ng/ml LIF, or Akt IV. There were no significant differences in Prdx4 mRNA expression between any of the normoxic groups. Following OGD exposure, LIF-treated cells showed a significant increase in Prdx4 mRNA (*P<0.05, significant from OGD), and parallel cultures co-incubated with Akt IV showed Prdx4 mRNA levels that were significantly lower than cells treated with LIF alone (#P<0.05, significant from OGD+LIF). n=3 independent cultures. Norm, normoxia. OGD, oxygen glucose deprivation. LIF, leukemia inhibitory factor. Akt IV, Akt Inhibitor IV.