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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Dev Biol. 2014 Aug 28;395(1):84–95. doi: 10.1016/j.ydbio.2014.08.026

Figure 5. Pex10CY/CY diaphragms display defects in axon extension and Schwann cell placement.

Figure 5

A, B, D, E. E15.5 (A, B) and E18.5 (D, E) wildtype and Pex10CY/CY diaphragms labeled with NF-M/SV2 (axons and pre-synaptic sites) and α-BTX (post-synaptic AChR clusters). C. Quantification as a percentage of the region of the diaphragm with AChR clusters in E15.5 and E18.5 wildtype and Pex10CY/CY diaphragms. F. Quantification of axon extension beyond the AChR clusters in E15.5 and E18.5 Pex10CY/CY diaphragms. G, H. E18.5 wildtype (G) and Pex10CY/CY (H) diaphragms labeled with NF-M (axons), α-BTX (post-synaptic AChR clusters) and SOX10 (Schwann cell nuclei, green). I. Quantification of percentage of nerves with SOX10 positive nuclei at the terminal end of the nerve. J. Quantification of SOX10 positive nuclei along the length of the nerve. K. Quantification of SOX10 positive nuclei adjacent to AChR clusters. A, B, D, E. Scale bar=200µm. G-H Scale bar=50µm. Asterisks indicate a significant difference (p< 0.005; t-test).