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. 2014 Oct 8;9(10):e109273. doi: 10.1371/journal.pone.0109273

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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PBMC were stimulated for 5 hours with TLR-agonists, and simultaneously treated with Brefeldin A. Cells were immuno-stained with antibodies against surface markers as demonstrated in Figure 1, then intracellularly stained to detect TNF-alpha production. Numbers in plots represent percentage of CD4⁺ DC (A), CD11c⁺ DC (B), and CD11c⁻ DC (C) producing TNF-alpha. Plots are representative of one animal. Graphs (D) show TNF-alpha production by DC subsets in 4 animals. Data are representative of two independent experiments. Error bars represent standard deviation.