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. 2014 Oct 8;9(10):e109273. doi: 10.1371/journal.pone.0109273

Figure 6. Type I IFN production by FACS purified peripheral blood DC subsets.

Figure 6

FACS purified DC subsets and whole PBMC were stimulated with R848 or media control for 20 hours. The supernatant was assessed for the presence of type I IFN by using a Mx-CAT reporter assay. Briefly, in the presence of type I IFN, the type I IFN-inducible Mx promoter would drive the transcription of chloramphenicol acetyltransferase (CAT). CAT protein levels are then detected by an ELISA assay. CAT expression indicated by absorbance at 405 nm (A), and calculated type I IFN levels (B) are shown.