Table 2. Antibody Panel for Intracellular Cytokine Staining.
Antibody | Isotype | Clone | Source/Catalog # |
TNF-a biotin | IgG2a | CC328 | AbD Serotec, Raleigh, NC |
CD11c | IgM | BAQ153A | WSU, Pullman, WA, |
CD3 | IgG1 | MM1A | WSU, Pullman, WA |
CD14 | IgG1 | MM61A | WSU, Pullman, WA |
CD11b | IgG1 | MM12A | WSU, Pullman, WA |
sIgM | IgG1 | IL-A30 | AbD Serotec, Raleigh, NC |
CD4 FITC | IgG2a | CC8 | AbD Serotec, Raleigh, NC |
PE-Cy7 IgM –secondary | Rat anti-mouse | II/41 | eBiosciences, San Diego, CA |
APC Cy7 IgG1–secondary | Goat ant-mouse | Southern Biotech, Birmingham, Alabama | |
BV421Streptavidin – secondary | Biolegend, San Diego, CA | ||
LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit | Life Technologies, Grand Island, NY |