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. 2014 Oct 8;9(10):e109525. doi: 10.1371/journal.pone.0109525

Figure 6. Manipulation of miR-155 does not significantly alter TNFα or IL-6 secretion triggered by F. tularensis infection.

Figure 6

A. Effects of LVS and Schu S4 on TNFα mRNA expression were analyzed by qRT-PCR. Data shown are the mean ±SEM (n = 3) and were normalized to GAPDH. B. MDMs were infected with LVS or Schu S4, and secreted TNFα and IL-6 were quantified at the indicated time points by ELISA. Data shown are the mean ±SEM (n = 3). C–D. MDMs were transfected with the indicated pre-miRs and anti-miRs 48 h prior to infection with LVS or Schu S4, as indicated. At 24 h post-infection the amount of TNFα (C) and IL-6 (D) secreted into the extracellular medium was quantified by ELISA. Data shown are the mean ±SEM (n = 3).