Table 3. Apparent steady-state kinetic constants of wild-type and mutant POx with D-glucose as electron donor (100 mM) and 1,4-BQ (varied from 0.01–0.5 mM) as electron acceptor.

	1,4-BQ-mediated dehydrogenase activity
Variant	K m (mM)	k cat (s−1)	k cat/K m (mM−1 s−1)	Relative k cat/K m (%)
WT	0.24±0.03*	152±6*	632±83*	100
T166R	0.14±0.02	166±11	1213±191	192
Q448H	0.057±0.007	12±1	203±30	32
L545C	0.042±0.006	110±4	2608±384	413
L547R	0.018±0.003	26±1	1465±251	232
N593C	0.14±0.02	4.6±0.2	32±5	5.1

*data from reference [47].