. 2014 Oct 8;9(10):e109242. doi: 10.1371/journal.pone.0109242

Table 4. Apparent steady-state kinetic constants of wild-type and mutant POx with D-glucose as electron donor (100 mM) and Fc⁺ (varied from 0.005–0.5 mM) as electron acceptor.

	Fc⁺-mediated dehydrogenase activity
Variant	K _m (mM)	k _cat (s⁻¹)	k _cat/K _m (mM⁻¹s⁻¹)	Relative k _cat/K _m (%)
WT^*	0.25±0.10^*	151±35^*	592±274^*	100
T166R	1.6±0.4	442±79	281±86	48
Q448H	0.56±0.21	27±7	48±22	8.1
L545C	0.12±0.01	142±4	1193±105	202
L547R	0.23±0.01	134±3	584±29	98
N593C	0.81±0.20	22±4	27±8	4.6

*data from reference [47].

Table 4. Apparent steady-state kinetic constants of wild-type and mutant POx with D-glucose as electron donor (100 mM) and Fc+ (varied from 0.005–0.5 mM) as electron acceptor.