Table 1.
Primary antibodies used in the immunohistochemistry
| Antigen (clone) | Location | Antibody species | Manufacturer (product) | Antigen-retrieval solution | Dilution | Internal positive control [reference No] |
|---|---|---|---|---|---|---|
| EpCAM (ESA) | M | Mouse monoclonal | Dako (M3525) | PH6 ciltrate buffer | 1:200 | Epithelium of pancreatic ducts, acinar cells and islets of Langerhans cells [29] |
| CD24 | M | Mouse monoclonal | Neomarkers (MS-1279) | PH6 ciltrate buffer | 1:50 | Acinar cells [27] |
| CD44 | M | Mouse monoclonal | Abcam(ab51037) | PH6 ciltrate buffer | 1:50 | Acinar cells [28] |
| CD133 | M | Rabbit polyclonal | Abnova(12663) | PH9 Tris EDTA buffer | 1:50 | Acinar cells [19] |
| CXCR4 | M | Mouse monoclonal | Zymed(35-8800) | PH6 ciltrate buffer | 1:50 | Acinar cells [17] |
| ALDH1 | C | Mouse monoclonal | Abcam(ab52492) | PH6 ciltrate buffer | 1:100 | Acinar cells and islets of Langerhans cells [19] |
M: membrane C: cytoplasm.