Table 1. Antibodies for immunohistochemistry.
Antibody | Clone | Company | Pre-treatment | Dilution | Positive control |
REG4 | mAb | In-house | Tris-HCl (pH 8.5) | 1∶50 | Colon |
MUC1 | mAb, Ma552 | Novocastra, UK | Citrate (pH 6.0) | 1∶25 | Stomach |
MUC2 | mAb, Ccp58 | Novocastra, UK | Citrate (pH 6.0) | 1∶100 | Colon |
MUC4 | mAb, 1G8 | Invitrogen,USA | Tris-EDTA (pH 9.0) | 1∶100 | Colon |
MU5AC | mAb, CLH2 | Novocastra, UK | Citrate (pH 6) | 1∶50 | Stomach |
Synaptophysin | mAb 27G12 | Novocastra, UK | Tris-EDTA (pH 9.0) | 1∶200 | Colon |
Chromogranin | mAb, 5H7 | Novocastra, UK | Tris-EDTA (pH 9.0) | 1∶2000 | Colon |
REG4 antibody described in detail in reference [20] mAb = monoclonal antibody. Antibody host: mouse.