Table 1. Antibodies for immunohistochemistry.

Antibody	Clone	Company	Pre-treatment	Dilution	Positive control
REG4	mAb	In-house	Tris-HCl (pH 8.5)	1∶50	Colon
MUC1	mAb, Ma552	Novocastra, UK	Citrate (pH 6.0)	1∶25	Stomach
MUC2	mAb, Ccp58	Novocastra, UK	Citrate (pH 6.0)	1∶100	Colon
MUC4	mAb, 1G8	Invitrogen,USA	Tris-EDTA (pH 9.0)	1∶100	Colon
MU5AC	mAb, CLH2	Novocastra, UK	Citrate (pH 6)	1∶50	Stomach
Synaptophysin	mAb 27G12	Novocastra, UK	Tris-EDTA (pH 9.0)	1∶200	Colon
Chromogranin	mAb, 5H7	Novocastra, UK	Tris-EDTA (pH 9.0)	1∶2000	Colon

REG4 antibody described in detail in reference [20] mAb = monoclonal antibody. Antibody host: mouse.